Details

Autor(en) / Beteiligte

• Müller, Hans Werner,

Titel

Neural degeneration and repair : gene expression profiling, proteomics, and systems biology [electronic resource]

Link zum Volltext

• Wiley Online Library - AutoHoldings Books

Beschreibungen/Notizen

• Description based upon print version of record.
• Includes bibliographical references and index.
• Neural Degeneration and Repair; Contents; Preface; List of Contributors; 1 Microarrays in Systems Neurobiology and Translational Neuroscience - From Genome Research to Clinical Applications; 1.1 Introduction; 1.2 Gene Expression Before Microarrays; 1.2.1 High-Throughput Gene Expression Techniques; 1.2.2 Contemporaneous Alternatives to Microarrays; 1.2.3 Microarray Technologies; 1.2.3.1 One-Color Oligonucleotide Arrays; 1.2.3.2 Two-Color Arrays; 1.2.3.3 Bead-Based Arrays; 1.3 Designing and Implementing a Microarray Experiment - From Start to Finish
• 1.3.1 Choosing the Proper Microarray Platform1.3.2 Preparing the Tissue for Hybridization; 1.3.3 Single-Cell Assays and Tissue Heterogeneity; 1.3.4 Microarray Hybridization and Scanning; 1.3.5 Preprocessing; 1.3.6 Gene Expression Analyses; 1.3.7 Analytical Challenges; 1.3.8 Validating Results; 1.4 Clinical Applications; 1.4.1 Neurological Disease-Relevant Research; 1.4.1.1 Incipient Alzheimer's Disease; 1.4.1.2 Using Animal Models to Study Neurodegeneration; 1.4.1.3 Determining Gene Pathways Using Meta-Analysis; 1.4.2 Cancer Research; 1.4.2.1 Tumor Classification and Patient Diagnosis
• 1.4.2.2 Determining Patient Prognosis1.4.2.3 Predicting Therapeutic Response to Treatment; 1.4.2.4 Determining Therapeutic Targets for Cancer; 1.4.3 Using Peripheral Tissues as a Substitute for Brain Tissue; 1.4.3.1 Gene Expression Profiling of Disease; 1.4.3.2 Dividing Complex Phenotypes into Subtypes Using Microarrays; 1.4.3.3 Applying Blood Classification to Treatments; 1.4.3.4 Advantages and Disadvantages of Using Blood Genomics for Brain Disorders; 1.4.3.5 Pure Cell Line Assays in Peripheral Blood; 1.4.4 Other Types of Microarrays in Clinical Setting
• 1.4.4.1 Gene Dose, Molecular Karyotyping, and Chromosomal Abnormalities1.4.4.2 SNP Genotyping and Beyond; 1.4.5 Future Clinical Applications: Pharmacogenomics; References; 2 A Meta-Analysis of Large-Scale Gene Expression Studies of the Injured PNS: Toward the Genetic Networks That Govern Successful Regeneration; 2.1 Introduction; 2.2 Lesion Models in the Peripheral Nervous System; 2.2.1 Sciatic Nerve; 2.2.2 Facial Nerve; 2.2.3 Postganglionic Trunk of the Superior Cervical Ganglion; 2.2.4 Injury Types; 2.3 Cellular Events After Injury
• 2.3.1 Regeneration After Dorsal Root Lesion is Limited Due to a Weak Neuronal Response to Axotomy2.3.2 Injury Signals; 2.3.3 Regeneration-Associated Gene Expression; 2.4 Large-Scale Screening Efforts to Find Novel Regeneration-Associated Genes; 2.4.1 Global Gene Expression Changes After Neuronal Injury Found by Gene Expression Profiling Studies; 2.4.2 Biological Processes Affected by Peripheral Nerve Injury; 2.4.3 Identification of Novel Regeneration-Associated Genes by Microarray Analysis
• 2.5 Developing New Insights into the Molecular Biology of Regenerative Neurite Outgrowth: From Single Gene to Genetic Networks
• With contributions from leading scientists around the world, this is the first book focussing on the analysis of nerve cell damage and repair using genomics, transcriptomics, proteomics and systems biology in order to develop novel therapeutic and diagnostic approaches for neural diseases. Following an introduction into the microarray technology in translational neuroscience, the book goes on to look at the use of '-omics' technologies to analyse molecular changes in traumatic injury, neuron degeneration and regeneration, oxidative stress response, neuropathic pain manifestation etc. The w
• English